Enzymes and Inhibitors
American Research Products Inc SRF Transcription Factor Activity Assay
SRF Transcription Factor Activity Assay
Ubpbio Recombinant human UbE1 from SF9 insect cells, no tag
UBE1 is an E1 enzyme within the E1, E2, E3 cascade which conjugates Ub to protein substrates. UBE1 activates Ub by catalyzing an ATP – dependent reaction; Ub is conjugated onto the catalytic cysteine residue of UBE1 by formation of a thioester bond. The activated Ub is then passed on to a Ub-conjugating enzyme E2; Ub charged E2 binds a Ub ligase E3 that directly binds substrate proteins. Ub is transferred to the HECT domain E3s prior to being conjugated on substrate proteins. For RING domain E3s, Ub is transferred directly from an E2 to substrate proteins.
ACROBiosystems Endoglycosidase H (Endo H) is a recombinant glycosidase cloned from Streptomyces plicatus and overexpressed in E. coli. Endo H cleaves the chitobiose core of high-mannose oligosaccharides and a limited number of hybrid oligosaccharides from
Endoglycosidase H is a recombinant glycosidase which cleaves within the chitobiose core of high mannose and some hybrid oligosaccharides from N-linked glycoproteins. It is a recombinant glycosidase cloned from Streptomyces plicatus and overexpressed in E. coli.
Ankom Technology ALPHA AMYLASE 250ML
Alpha Amylase 250 ml. ANKOM FAA is a starch-hydrolyzing alpha-amylase enzyme with high heat and pH stability derived from a genetically modified strain of Bacillus licheniformis. Used for liquification of starch during Neutral Detergent Fiber Analysis.
Immunology Consultants Hamster (CHO) Lysosomal Acid Lipase - Recombinant, 0.025mg
Recombinant CHO Lysosomal Acid Lipase (LAL; NCBI XP_035297776.1) is expressed in CHO cells with a 6xHIS tag and purified to >95% purity. LAL appears at ~55 kDa under reducing conditions and is quantified by A280. As a lipid‑metabolizing lysosomal enzyme, LAL can co‑purify with biologics and contribute to product degradation during purification or storage. This high‑quality recombinant antigen provides a reliable standard for ELISA development, antibody validation, and HCP profiling. Its CHO‑derived structure ensures biological relevance for impurity monitoring and process optimization. A valuable tool for teams studying lipid‑processing enzymes, assessing LAL‑related risks, or refining HCP detection workflows.
New England Biolabs, Inc. ScaI-HF – 5000 units
A high fidelity restriction endonuclease that recognizes the sequence AGT^ACT. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
Research Products International Corp X-GAL and IPTG Ready to Use Non-Toxic Solution 10mg/ml, 500 Milligrams
Stable solution of X-GAL and IPTG. Does not contain DMSO or DMF. Solution concentration is 10 mg/ml X-Gal and 8 mg/ml IPTG.
Chromogenic substrate of β-Galactosidase used in combination with IPTG for detection of β-Galactosidase activity in bacterial colonies. X-GAL is cleaved at the B1-4 bond between galactose and the 5-Bromo-4-chloro-3-indolyl portion of X-GAL by β-Galactosidase via hydrolysis. The cleavage of X-GAL results in the production of a water insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies with vectors such as Lambda-11, M13mp18 and 19, pUC18 and 19, pUR222 the E. coli lacZ gene is transformed to lac+ cells. WARNING! This product can expose you to 1-Methyl-2-Pyrrolidon, a chemical which is known to the State of California to cause developmental harm. For more information go to www.P65Warnings.ca.gov.
Vector Biolabs SRF-Luc Adenovirus (Ad-SRF-Luc)
Ready to use in vivo grade AAV and Adenovirus Ready to use in vivo grade AAV and Adenovirus pre synthesized DNA vector High titer viral vectors 1/EA
Abcam 1,2-Dioleoyl-3-(pyren-1-yl) decanoyl-rac-glycerol, 1MG
MW 975.5 Da. Fluorescent triglyceride analog useful for measuring cholesteryl ester transfer and protein-mediated triacylglycerol transport between plasma lipoproteins. Popular substrate for detections of lipoprotein lipase and hepatic triacylglycerol lipases.
The product is subject to the following: Abcam Restricted Use Statement
Abcam Resorufin acetate, 25MG
MW 255.22 Da, Purity >98%. Red fluorogenic esterase substrate, useful in cell viability assays, or for high throughput screening of esterase activity in a live cell format.
The product is subject to the following: Abcam Restricted Use Statement
INTACT GENOMICS INC T7 Endonuclease I, 1,250 Units
T7 Endonuclease I is a stable homodimer of identical 149 amino acid subunits, produced from a recombinant gene in *E. coli*. It recognizes and cleaves mismatched DNA where the mismatched DNA length position is more than 1 bp. This enzyme is useful for quantitatively estimating the nuclease-induced mutation frequency of gene edited cells, which are generated by CRISPR/ZFN/TALEN at desired target sites, PCR-amplified, denatured, and re-annealed to form mismatched DNA.
- Resolves four-way junction or branched DNA.
- Detects or cleaves heteroduplex and nicked DNA.
- Randomly cleaves linear DNA for shot-gun cloning.
- Physical purity is ≥99% as assessed by SDS-PAGE with Coomassie® blue staining.
- Includes T7 Endonuclease I and 10x T7 Endonuclease I reaction buffer.
- Storage temperature: -20°C.
- Free from detectable contaminating nuclease activities.
SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES ANTI-TOPOISOMERASE I-DNA COVAL
NC3728455 ANTI-TOPOISOMERASE I-DNA COVAL